Induction of Heterothallic and Homothallic Zygotes in Dictyostelium discoideum by Ethylene

Gene expression during oosporogenesis in heterothallic and homothallic Phytophthora.

A large-scale screen for genes induced during sexual development was performed in the heterothallic oomycete Phytophthora infestans, the potato blight agent. Of 15,644 unigenes on an Affymetrix chip, 87 were induced >10-fold during mating, with 28 induced >100-fold. This was validated in independent matings using RNA blots and RT-PCR. Only 44 genes resembled sequences in GenBank. These encoded ...

Dictyostelium discoideum

Dictyostelium discoideum is a cellular slime mold [4] that serves as an important model organism [5] in a variety of fields. Cellular slime molds have an unusual life cycle. They exist as separate amoebae, but after consuming all the bacteria in their area they proceed to stream together to form a multicellular organism. These features make them a valuable tool for studying developmental proces...

influx in Dictyostelium discoideum

Thermotaxis by pseudoplasmodia of Dictyostelium discoideum.

The temperature dependence of migration rate and of the thermotactic sensitivity of pseudoplasmodia of Dictyostelium discoideum has been measured. Migration rate increases with temperature to 20 degrees, is temperature insensitive from 20 degrees to 27.5 degrees, and decreases with temperature to 29 degrees, above which point migration ceases. However, pseudoplasmodia formed from cells grown at...

Translocations in Dictyostelium discoideum.

Fourteen translocations of independent origin were identified in Dictyostelium discoideum on the basis of segregation anomalies of diploids heterozygous for these chromosome rearrangements, all of which led to the cosegregation of unlinked markers. Many of these translocations were discovered in strains mutagenized with MNNG or in strains carrying mutations affecting DNA repair; however, sponta...